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Following incubation, membranes were washed three times with TBST (5 min each) and incubated for 2 h at room temperature with alkaline phosphatase (AP)-conjugated secondary antibodies: goat anti-mouse (ab97020, Abcam 1:5000 dil.) or goat anti-rabbit (#ab6722, Abcam 1:3000 dil.), depending on the host species of the primary antibodies

10.1016/j.cardfail.2006.08.211

Results Prolonged GLP-1R Signalling Stimulates Glycolysis via Up-Regulation of Glycolytic Enzymes GLP-1 requires the presence of glucose for stimulation of insulin secretion 14

Acute Toxicity Mice, intraperitoneal LD50: 750 mg/kg, with no other details provided besides the lethal dose